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KMID : 1145120160140040427
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Asian Journal of Beauty and Cosmetology
2016 Volume.14 No. 4 p.427 ~ p.435
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Inhibitory Effects of Hericium erinaceus Extracts on Melanin Synthesis and Oxidative Stress
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Jeon See-Un
Hwang Won-Jung
Hong Yoon-Hee
Kim Mi-Jung
Ahn Eun-Sook
Park Se-Yeon
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Abstract
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Purpose: The aim of this research is to investigate the effects of Hericium erinaceus (H. erinaceus) extracts on melanin synthesis and regulation of oxidative stress.
Methods: H. erinaceus was extracted with 95% ethanol and distilled water. The ethanol extracts were further partitioned with distilled water and chloroform. Melanin contents were determined by measuring the absorbance at 450 nm in B16F1 melanoma cells. MTT assay was performed to check cytotoxicity of H. erinaceus extracts on B16F1 and HaCaT cells. Moreover, β-catenin/Tcf activity was measured by luciferase assay. The anti-oxidant effects were confirmed by DCFDA expression after TBHP treatment.
Results: Wnt3a, a ligand of Wnt signaling pathway, increased β-catenin signaling and melanin synthesis, while H. erinaceus extracts reduced melanin synthesis induced by Wnt3a in B16F1 melanoma cells. H. erinaceus extracts also inhibited β-catenin activity in a dose-dependent manner. In addition, these extracts showed anti-oxidant properties in oxidative stress-induced keratinocytes. H. erinaceus extracts showed little cytotoxicity in melanoma cells and keratinocytes used in this research.
Conclusion: H. erinaceus extracts might be useful as a safe and effective natural skin-whitening agent.
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KEYWORD
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Wnt/β-catenin, Hericium erinaceus, Extracts, Melanoma, Anti-oxidant
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